A lock-docking oligo(dT) primer for 5' and 3' RACE PCR.

Combination of direct DNA sequencing with degenerate primer-mediated PCR and 5'-/3'-RACE to screen novel cDNA sequences.

reaction without addition of primers. By lowering the total number of cycles in the two reactions, the risk of introducing mutations in the DNA sequence is reduced. The improvement of the method may be important for generation of genetic libraries where reduction of the overlap extension to one cycle, together with high assembly efficiency, may be crucial for preserving the diversity and mainta...

Biologically-generated primer for PCR: PCR primer of unknown sequence

We describe a method for producing specific PCR primers directly from PCR product, bypassing the usual need to know the primer sequence. Lack of abundance of primers derived from a PCR product is compensated for by the incorporation of an arbitrary 5'TAG sequence which acts as a surrogate template target for the bulk amplification phase. We use the technique to amplify clonospecific rearranged ...

T955D-V956I-pGex: Forward primer: 5’-AGACAATTCAGCAATCATACGATATTTGAG- TCGACTCGAGCGGCC-3’, Reverse primer: 5’-GGCCGCTCGAGTCGACTCAAATATC- GTATGATTGCTGAATTGTCT-3’; PMA2-T955D-V956I-pTYB12: Forward primer: 5’-G- ACAATTCAGCAATCATACGATATTTGAGTCGACGGCGGC-3’, Reverse primer:5’-G- CCGCCGTCGACTCAAATATCGTATGATTGCTGAATTGTC-3’; T14-3-3c-E19R: Forward primer: 5’-AACGTGTACATGGCAAAGCTTGCAAGGCAAGCTGAGAGG-3’, Reverse primer: 5’-CCTCTCAGCTTGCCTTGCAAGCTTTGCCATGTACACGTT-3’; T14-3-3c-E19K: Forward primer: 5’-ACGTGTACATGGCAAAGCTTGCAAAGCAAGCT-

Primer1: Primer Design Web Service for Tetra-Primer ARMS-PCR

Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given...

JAK-STAT pathway and JAK inhibitors: a primer for dermatologists

Background: All cellular events depend upon the DNA synthesis and gene expression involving complex interplay between ligands such as interleukins and interferons, with various cell membrane receptors. These ligand-receptors interactions transmit signals within the cell via numerous signal transduction pathways to affect gene expression. Janus kinase/signal transducer and activator of transcrip...